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KMID : 0880220140520070626
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Journal of Microbiology
2014 Volume.52 No. 7 p.626 ~ p.629
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Identification of high-specificity H-NS binding site in LEE5 promoter of enteropathogenic Esherichia coli (EPEC)
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Bhat Abhay Prasad
Shin Min-Sang
Choy Hyon-E.
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Abstract
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Histone-like nucleoid structuring protein (H-NS) is a small but abundant protein present in enteric bacteria and is involved in compaction of the DNA and regulation of the transcription. Recent reports have suggested that H-NS binds to a specific AT rich DNA sequence than to intrinsically curved DNA in sequence independent manner. We detected two high-specificity H-NS binding sites in LEE5 promoter of EPEC centered at -110 and -138, which were close to the proposed consensus H-NS binding motif. To identify H-NS binding sequence in LEE5 promoter, we took a random mutagenesis approach and found the mutations at around -138 were specifically defective in the regulation by H-NS. It was concluded that H-NS exertsmaximumrepression via the specific sequence at around -138 and subsequently contacts a subunit of RNAP through oligomerization.
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KEYWORD
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LEE5, H-NS, nucleation site, consensus motif, transcriptional repression, gene regulation, transcription
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